Oregano demonstrates distinct tumour-suppressive effects in the breast carcinoma model.

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Oregano demonstrates distinct tumour-suppressive effects in the breast carcinoma model.

2015-10-16-1445000635-9657874-knowledgeispower

Oregano demonstrates distinct tumour-suppressive effects in the breast carcinoma model.

Kubatka P1Kello M2Kajo K3Kruzliak P4Výbohová D5Mojžiš J2Adamkov M6Fialová S7Veizerová L8Zulli A9Péč M10Statelová D11Grančai D7,Büsselberg D12.

Author information

  • 1Department of Medical Biology, Jessenius Faculty of Medicine, Comenius University in Bratislava, Malá Hora 4, 03601, Martin, Slovakia. kubatkap@gmail.com.
  • 2Department of Pharmacology, Faculty of Medicine, P. J. Šafárik University, Kosice, Slovakia.
  • 3Department of Pathology, Slovak Medical University and St. Elisabeth Oncology Institute, Bratislava, Slovakia.
  • 4Laboratory of Structural Biology and Proteomics, Central Laboratories, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Palackeho tr. 1946/1, 612 42, Brno, Czech Republic. kruzliakpeter@gmail.com.
  • 5Department of Anatomy, Jessenius Faculty of Medicine, Comenius University in Bratislava, Martin, Slovakia.
  • 6Department of Histology and Embryology, Jessenius Faculty of Medicine, Comenius University in Bratislava, Martin, Slovakia.
  • 7Department of Pharmacognosy and Botany, Faculty of Pharmacy, Comenius University in Bratislava, Bratislava, Slovakia.
  • 8Department of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Toxicological and Antidoping Center, Comenius University in Bratislava, Bratislava, Slovakia.
  • 9The Centre for Chronic Disease Prevention and Management (CCDPM), College of Health and Biomedicine, Victoria University, Melbourne, VIC, Australia.
  • 10Department of Medical Biology, Jessenius Faculty of Medicine, Comenius University in Bratislava, Malá Hora 4, 03601, Martin, Slovakia. pec@jfmed.uniba.sk.
  • 11Clinic of Stomatology and Maxillofacial Surgery, Jessenius Faculty of Medicine, Comenius University in Bratislava, Martin, Slovakia.
  • 12Department of Physiology and Biophysics, Weill Cornell Medical College in Qatar, Qatar Foundation – Education City, Doha, Qatar.

Abstract

PURPOSE:

There has been a considerable interest in the identification of natural plant foods for developing effective agents againstcancer. Thus, the anti-tumour effects of oregano in the in vivo and in vitro breast cancer model were evaluated.

METHODS:

Lyophilized oregano (ORE) was administered at two concentrations of 0.3 and 3 % through diet. The experiment was terminated 14 weeks after carcinogen administration. At autopsy, mammary tumours were removed and prepared for histopathological and immunohistochemical analysis. Moreover, in vitro evaluation in MCF-7 cells was carried out.

RESULTS:

Low-dose ORE suppressed tumour frequency by 55.5 %, tumour incidence by 44 %, and tumour volume by 44.5 % compared to control animals. Analysis of rat tumour cells showed Ki67, VEGFR-2, CD24, and EpCAM expression decrease and caspase-3 expression increase after low-dose ORE treatment. High-dose ORE lengthened tumour latency by 12.5 days; moreover, Bcl-2, VEGFR-2, CD24, and EpCAM expression decrease and caspase-3 expression increase in carcinoma cells were observed. Histopathological analysis revealed a decrease in the ratio of high-/low-grade carcinomas in both treated groups. In vitro studies showed that ORE decreased survival and proliferation of MCF-7 cells. In ORE-treated MCF-7 cells, an increase in cells expressing sub-G 0/G 1 DNA content and an increase in the percentage of annexin V/PI positive MCF-7 cells were observed. In vitro, both caspase-dependent and possible non-caspase-dependent apoptotic pathways were found. The deactivation of anti-apoptotic activity of Bcl-2, a decrease in mitochondrial membrane potential, and the activation of mitochondrial apoptosis pathway were observed in the ORE-treated MCF-7 cells.

CONCLUSIONS:

Our results demonstrate, for the first time, a distinct tumour-suppressive effect of oregano in the breast cancer model.

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